Beschreibung
InhaltsangabeMASS SPECTROMETRY OF AMINO ACIDS AND PROTEINS Introduction Basic Protein Chemistry and How it Relates to MS Sample Preparation and Data Acquisition Data Analysis of LC-MS/MS (or CE-MS/MS) of Mixtures MS of Protein Structure, Folding, and Interactions Conclusions and Perspectives XRAY STRUCTURE DETERMINATION OF PROTEINS AND PEPTIDES Introduction Growing Crystals Symmetry and Space Groups XRay Scattering and Diffraction Collecting and Processing Diffraction Data Solving the Structure (Determining Phases) Analyzing and Refining the Structure NUCLEAR MAGNETIC RESONANCE OF AMINO ACIDS, PEPTIDES, AND PROTEINS Introduction Amino Acids Peptides Proteins Conclusions STRUCTURE AND ACTIVITY OF N-METHYLATED PEPTIDES Introduction Conformational Effects of N-Methylation Effects of NMethylation on Bioactive Peptides Concluding Remarks HIGHPERFORMANCE LIQUID CHROMATOGRAPHY OF PEPTIDES AND PROTEINS Introduction Basic Terms and Concepts in Chromatography Chemical Structure of Peptides and Proteins HPLC Separation Modes in Peptide and Protein Analysis Method Development from Analytical to Preparative Scale Illustrated for HP-RPC Multidimensional HPLC Conclusions LOCAL SURFACE PLASMON RESONANCE AND ELECTROCHEMICAL BIOSENSING SYSTEMS FOR ANALYZING FUNCTIONAL PEPTIDES Localized Surface Plasmon Resonance (LSPR)-Based Microfluidics Biosensor for the Detection of Insulin Peptide Hormone Electrochemical LSPR-Based Label-Free Detection of Melittin LabelFree Electrochemical Monitoring of BetaAmyloid (ABeta) Peptide Aggragation SURFACE PLASMON RESONANCE SPECTROSCOPY IN THE BIOSCIENCES Introduction SPRBased Optical Biosensors Principle of Operation of SPR Biosensors Description of a SPR Instrument Application of SPR in Immunosensor Design Application of SPR in Membrane Interactions Data Analysis Conclusions ATOMIC FORCE MICROSCOPY OF PROTEINS Foreword AFM Bioimaging Highlights Issues Force Measurements Liquid Imaging Sample Preparation for Bioimaging Outlook SOLVENT INTERACTIONS WITH PROTEINS AND OTHER MACROMOLECULES Introduction Solvent Applications Solvent Application for Viruses Solvent Application for DNA Mechanism ProteinSolvent Interactions in Frozen and FreezeDried Systems Conclusions ROLE OF CYSTEINE Sulfur: A Redox Chameleon with Many Faces Three Faces of Thiols: Nucleophilicity, Redox Activity, and Metal Binding Towards a Dynamic Picture of Disulfide Bonds Chemical Protection and Regulation via S-Thiolation "Dormant" Catalytic Sites Peroxiredoxiin/Sulfiredoxin Catalysis and Control Pathway Higher Sulfur Oxidation States: From the Shadows to the Heart of Biological Sulfur Chemistry Cysteine as a Target for Oxidants, Metal Ions, and Drug Molecules Conclusions and Outlook ROLE OF DISULFIDE BONDS IN PEPTIDE AND PROTEIN CONFORMATION Introduction Probing the Role of Disulfide Bonds Contribution of Disulfide Bonds to Protein Stability Role of Disulfide Bonds in Protein Folding Role of Individual Disulfide Bonds in Protein Structure Disulfide Bonds in Protein Dynamics Disulfide Bonding Patterns and Protein Topology Applications Conclusions QUANTITATIVE MASS SPECTROMETRY-BASED PROTEOMICS Introduction Quantification in Biological MS Indentifying Proteins Interacting with Small Molecules with Quantitative Proteomics Conclusions TWODIMENSIONAL GEL ELECTROPHORESIS AND PROTEIN/POLYPEPTIDE ASSIGNMENT Introduction Aim of Protein Analysis and Development of 2-DE Techniques Current Status of 2-DE Techniques Development of Protein Assignment Techniques on 2-DE Gels and Current Status of Mass Spectrometric Techniques Conclusions BIOINFORMATICS TOOLS FOR DETECTING POST-TRANSLATIONAL MODIFICATIONS IN MASS SPECTROMETRY DATA Introduction PTM Discovery with MS Database Resources for PTM Analysis Conclusions
Autorenportrait
InhaltsangabeMASS SPECTROMETRY OF AMINO ACIDS AND PROTEINS Introduction Basic Protein Chemistry and How it Relates to MS Sample Preparation and Data Acquisition Data Analysis of LC-MS/MS (or CE-MS/MS) of Mixtures MS of Protein Structure, Folding, and Interactions Conclusions and Perspectives XRAY STRUCTURE DETERMINATION OF PROTEINS AND PEPTIDES Introduction Growing Crystals Symmetry and Space Groups XRay Scattering and Diffraction Collecting and Processing Diffraction Data Solving the Structure (Determining Phases) Analyzing and Refining the Structure NUCLEAR MAGNETIC RESONANCE OF AMINO ACIDS, PEPTIDES, AND PROTEINS Introduction Amino Acids Peptides Proteins Conclusions STRUCTURE AND ACTIVITY OF N-METHYLATED PEPTIDES Introduction Conformational Effects of N-Methylation Effects of NMethylation on Bioactive Peptides Concluding Remarks HIGHPERFORMANCE LIQUID CHROMATOGRAPHY OF PEPTIDES AND PROTEINS Introduction Basic Terms and Concepts in Chromatography Chemical Structure of Peptides and Proteins HPLC Separation Modes in Peptide and Protein Analysis Method Development from Analytical to Preparative Scale Illustrated for HP-RPC Multidimensional HPLC Conclusions LOCAL SURFACE PLASMON RESONANCE AND ELECTROCHEMICAL BIOSENSING SYSTEMS FOR ANALYZING FUNCTIONAL PEPTIDES Localized Surface Plasmon Resonance (LSPR)-Based Microfluidics Biosensor for the Detection of Insulin Peptide Hormone Electrochemical LSPR-Based Label-Free Detection of Melittin LabelFree Electrochemical Monitoring of BetaAmyloid (ABeta) Peptide Aggragation SURFACE PLASMON RESONANCE SPECTROSCOPY IN THE BIOSCIENCES Introduction SPRBased Optical Biosensors Principle of Operation of SPR Biosensors Description of a SPR Instrument Application of SPR in Immunosensor Design Application of SPR in Membrane Interactions Data Analysis Conclusions ATOMIC FORCE MICROSCOPY OF PROTEINS Foreword AFM Bioimaging Highlights Issues Force Measurements Liquid Imaging Sample Preparation for Bioimaging Outlook SOLVENT INTERACTIONS WITH PROTEINS AND OTHER MACROMOLECULES Introduction Solvent Applications Solvent Application for Viruses Solvent Application for DNA Mechanism ProteinSolvent Interactions in Frozen and FreezeDried Systems Conclusions ROLE OF CYSTEINE Sulfur: A Redox Chameleon with Many Faces Three Faces of Thiols: Nucleophilicity, Redox Activity, and Metal Binding Towards a Dynamic Picture of Disulfide Bonds Chemical Protection and Regulation via S-Thiolation "Dormant" Catalytic Sites Peroxiredoxiin/Sulfiredoxin Catalysis and Control Pathway Higher Sulfur Oxidation States: From the Shadows to the Heart of Biological Sulfur Chemistry Cysteine as a Target for Oxidants, Metal Ions, and Drug Molecules Conclusions and Outlook ROLE OF DISULFIDE BONDS IN PEPTIDE AND PROTEIN CONFORMATION Introduction Probing the Role of Disulfide Bonds Contribution of Disulfide Bonds to Protein Stability Role of Disulfide Bonds in Protein Folding Role of Individual Disulfide Bonds in Protein Structure Disulfide Bonds in Protein Dynamics Disulfide Bonding Patterns and Protein Topology Applications Conclusions QUANTITATIVE MASS SPECTROMETRY-BASED PROTEOMICS Introduction Quantification in Biological MS Indentifying Proteins Interacting with Small Molecules with Quantitative Proteomics Conclusions TWODIMENSIONAL GEL ELECTROPHORESIS AND PROTEIN/POLYPEPTIDE ASSIGNMENT Introduction Aim of Protein Analysis and Development of 2-DE Techniques Current Status of 2-DE Techniques Development of Protein Assignment Techniques on 2-DE Gels and Current Status of Mass Spectrometric Techniques Conclusions BIOINFORMATICS TOOLS FOR DETECTING POST-TRANSLATIONAL MODIFICATIONS IN MASS SPECTROMETRY DATA Introduction PTM Discovery with MS Database Resources for PTM Analysis Conclusions
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